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The "Slider" below describes the analysis process with screen captures from the Oligo Maker app. Refresh the page if slider is not moving on it's own.
Oligo type is chosen & oligo sequence entered
The mRNA and oligo concentrations are set
The default settings are 50 pm and 1 uM respectively
Salt concentration set at 1 M
Default for RNA and DNA only
Oligo Name and Description entered
Data includes hairpins and dimers
(highest energy interactions calculated)
Oligos are given a grade!
green=GREAT, yellow=OK, red=BAD
Click 'Save' in the upper right corner to save all the data
The 'Saved' tab shows the list of saved analyses or designs
We can enter the target sequence
Then we can enter mismatches in our oligo sequence to see the impact!
We introduced 5 mismatches into our oligo sequence.
The oligo's Tm dropped 23 degrees - now marked in red!
The Oligio Grade went down but still in the green (barely).
We saved the mismatch oligo data too!
With a click we can send you an email with ALL the data you need!
Click to view the Oligo Design "slider" presentation