The "Slider" below describes the analysis process with screen captures from the Oligo Maker app. Refresh the page if slider is not moving on it's own.

Oligo type is chosen & oligo sequence entered

The mRNA and oligo concentrations are set

The default settings are 50 pm and 1 uM respectively

Salt concentration set at 1 M

Default for RNA and DNA only

Oligo Name and Description entered

'Analyze' tapped

Analysis Output

Data includes hairpins and dimers

(highest energy interactions calculated)

Oligos are given a grade!

green=GREAT, yellow=OK, red=BAD

Click 'Save' in the upper right corner to save all the data

The 'Saved' tab shows the list of saved analyses or designs

We can enter the target sequence

Then we can enter mismatches in our oligo sequence to see the impact!

We introduced 5 mismatches into our oligo sequence.

click 'Analyze'

The oligo's Tm dropped 23 degrees - now marked in red!

The Oligio Grade went down but still in the green (barely).

We saved the mismatch oligo data too!

With a click we can send you an email with ALL the data you need!

Click to view the Oligo Design "slider" presentation